Hedera helix is a plant that is valued for its medicinal properties and on the other hand criticized for its invading potential. Beside its medicinal use, a wide utilization as decorative plant is typical for this specie. Up-to date only a few molecular data are available for Hedera helix genome, thus the aim of the study was to evaluate the utilization of different DNA markers for the its further population studies purposes. In total, eight different DNA marker techniques were used for the PCR analysis – iPBS, BARE1 IRAP, Cassandra IRAP, Tst1 IRAP, HACRE 1 IRAP, RAPD, ISSR and miRNA. In total, 38 different iPBS primers were tested with the best results for primers 2152, 2270, 2079, 1899 and 2033. In the case of RAPD primers, all of them worked in the PCR excellent. For ISSR, only a 42 % of the used primers provided results that vere evaluated as very good or excellent ISSR profile. The most successful results were obtained by microsatelite repetitions (GA)8T, (CTG)3GC and (TG)10. A novel type of DNA markers – miRNA provided results that are promising for Hedera helix, L. population studies for their further utilization.