Amygdalin is a cyanogenic glucoside initially obtained from the seeds of bitter almonds (Prunus dulcis). It is a natural product that owns antitumor activity and has also been used for the treatment of asthma, bronchitis, emphysema, leprosy and diabetes. It is composed of one molecule of benzaldehyde, two molecules of glucose and one molecule of hydrocyanic acid. Cyanide is not cancerocidal as long as glucose is available. The present in vivo study was designed to reveal whether amygdalin is able to cause changes in the energetic profile of rabbit as a biological model. A 12 adult male rabbits were randomly divided into three groups: the control group without any amygdalin administration and two experimental groups receiving daily intramuscular injections (IM) of amygdalin at 0.6 and 3.0 mg/kg b.w. respectively over the period of 28 days. The body weight of each experimental animal was recorded weekly during the whole study. Serum levels of energetic profile (glucose, triglycerides and cholesterol) were determined. Intramuscular application of amygdalin did not affect (P≥0.05) the serum levels of none of selected biochemical parameters significantly. In this in vivo study, no obvious beneficial or negative effects of amygdalin on energetic profile of male rabbits were demonstrated.