Mária Labajová*, Slavomíra Senková, Jana Žiarovská, Katarína Ražná, Milan Bežo, Veronika Štefúnová, Lucia Zeleňáková
Biotechnology of Biotechnology
Amaranth is one of the re-discovered crops. Amaranth DNA polymorphism changes caused by γ-radiation in selected amaranth lines - Amaranthus cruenthus L. were observed using genotypes Ficha and Hybrid K-433 (the result of interspecific hybridization of species A. hypochondriacus A. hybridus). Mutant lines are M8 generation positively selected plants for weight of thousand seeds. To determine the polymorphism between individual samples ISSR technique was used. Primer Ama1 detected interspecific and primer Ama2 revealed intra – and interspecific polymorphisms, too. Primer Ama1 distinguished only the cultivar Ficha and hybrid K-433 accessions. Primer Ama2 created more polymorphic DNA profiles and has the ability to distinguish not only Ficha cultivar and hybrid K-433, but mutant accessions among themselves. A total of 11-13 band levels for the primer Ama1 and 14-16 for the primer Ama2 were obtained.
Amaranth, ISSR, inter- and intra specific variability