Barbara Mickowska, Peter Socha, Dana Urminská*, Ewa Cieślik
Biotechnology of Biotechnology
The aim of this study was to compare the prolamin complex of several varieties of cereals: 16 varieties of wheat (including common, durum and spelt wheat), 8 varieties of barley, 3 varieties of triticale and 1 variety of rye. In amino acids composition the major part represent glutamic acid in all type of prolamins (38 – 43 %) but there were some differences between content of proline (in wheat and triticale it was 17 %, in rye 20 % but in barley
25 %). By ELISA based on monoclonal antibody R5 it was showed positive reaction in relation to coeliac disease active peptides. Immunoblot based on polyclonal gluten antibody detected only proteins with molecular weight higher than 35 kDa.
Cereals; Prolamins; Amino Acid; ELISA; Western blot