THE CYTOTOXIC EFFECT OF ESSENTIAL OILS CITRUS AURANTIUM PEELS ON HUMAN COLORECTAL CARCINOMA CELL LINE (LIM1863)

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June – July 2012, vol. 1, no. 6
pages: 1476-1487
Article type: Biotechnology of Biotechnology
Abstract: Citrus essential oils (EOs) contain different terpens that have been shown to possess antitumor effects. We determined the cytotoxic effect of essential oils of Citrus aurantium L. subspamara peels on a colorectal cancer cell line (Lim1863). Three samples were harvested from three locations in Syria. EOs were extracted by hydrodistilation and analyzed by GC-MS. EOs content of Limonene was96-97.7 % while α-pinene and β-myrcenewere0.35-1% and 0.9-1.4% respectively. Various concentrations of EOs (0.25-48 µl/ml) were added to cultured cells and incubated for 72 h. Cell viability was evaluated using the MTT-based cytotoxicity assay. The obtained IC50 value range of C. aurantium Eos was 2.18-2.44 µl/ml. In conclosion, C. aurantium peels Eos obviously reduced the cell viability and it might have cytotoxic effect against colorectal cancer cell line.
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