Abstract: In order to study canola (Brassica napus L.) in vitro seedling growth after seed imbibition by human recombinant interferon alpha 2b (INF) solution, the measurements of fresh weight (FW), total soluble protein (TSP) content, and superoxide dismutase (SOD) activity were conducted in 7-day-old plantlets. INF applications did not improve canola seed germination. However, FW of seedlings increased by 100% (102 IU/ml INF), 140% (103 IU/ml INF), and 70% (104 IU/ml INF) in comparison with controls (treatments by pure water, INF after boiling, or INF with diethyldithiocarbamate). Increment of FW was detected in hypocotyls and roots. TSP content was not affected in cotyledons and decreased in hypocotyls. SOD activity increased up to 2.18-fold in cotyledons and 1.47-fold in hypocotyls. Human recombinant INF alpha 2b application caused an increase in canola seedling biomass (up to 2.4-fold) during in vitro growth and we suppose that it was regulated via increased SOD activity.