Recently focus on research has increased on natural products, new and useful compounds like antibiotics, chemotherapeutic agents, agrochemicals that are low toxic, highly effective and minor environment impact. The study was designed to screen the antibacterial potential of bacterial endophytes isolated from different medicinal plants against Gram positive and Gram negative human pathogenic bacteria. Endophytes were isolated by using standard protocols from three medicinal plants. Primary screening and secondary screening were done by perpendicular streaking and agar well diffusion method respectively to check antimicrobial property of endophytes. Antibiotic susceptibility test of pathogenic bacteria was determined by disc diffusion method against a number of antibiotics. After screening, it was found that FjR1 and FjF2 isolated from Flacourtia jangomas (Lour.) Raeusch showed potential antimicrobial activity against both Gram positive and Gram negative pathogenic bacteria. The present study shows that F. jangomas is a good source for bacterial endophytes which can be promising source for production of effective antimicrobial compound with pharmacological importance.