Abstract: The objective of the present study was to evaluate the effect of two probiotic strains Lactobacillus fermentum CCM 7158 and Enterococcus faecium M 74 given to the drinking water on the some parameters of energy profile (cholesterol, triglycerides, total protein, glucose) and antioxidant status (total antioxidant status – TAS, albumins, bilirubin) of female broiler chickens. The experiment was conducted on hybrid Hybro (n=180). The feeding period lasted 42 days. Experimental chickens of E1 group received a probiotic preparation in drinking water with concentration of 1.109 colony forming units (CFU) of L. fermentum CCM 7158 in 1 g of nutrient medium and experimental chickens of E2 group concentration of 2.109 CFU of E. faecium M-74 in 1 g of nutrient medium. The control group of animals received water without any additives. TAS in both probiotic groups was significantly increased. No significant differences were found in other parameters.