The galacto-oligosaccharides and Saccharomyces boulardii are very useful for the intestinal microbiota. The yeast acts as probiotic and used for the reduction of monosaccharides from GOS mixture. The present study was concerned with the production of synbiotic product from GOS and S. boulardii through transgalactosylation process. The GOS was produced from β-galactosidase (156 U/1 ml of lactose solution) of Kluyveromyces lactis using lactose (250g/L) as substrate at 37ºC. The GOS mixture was analyzed through thin layer chromatography and megazyme kit. The maximum production of GOS occurs after 5 hr at 37ºC with phosphate buffer (pH 6.5). The GOS mixture was treated with probiotic S. boulardii. The monosaccharides were reduced at 37ºC after 4 hrs using 300µl yeast/5 ml GOS mixture. Then synbiotic product was formed and undergoes lyophilization procedure. The total yeast count was 3.9 × 104CFU/g in lyophilized product. The in vitro bifidogenic effect was determined. Bifidobacterium shows more positive effect towards prebiotic and combined effect of prebiotic and probiotic as compared to Lactobacillus.