Abstracts Special issue on Animal Physiology, 2013, vol. 2, Abstracts special issue
Michal Miškeje, Tomáš Slanina, Ida Petrovičová, Peter Massányi
Biotechnology of Biotechnology
Viability of turkey semen is very important in the management of turkey reproduction. It can be influenced by many factors and additives. Very important from these factors is survival of spermatozoa in female oviduct. Target of our study was to analyze the effect of different concentrations of extracts from fallopian tubes (oviducts) on the motility parameters of turkey spermatozoa in vitro at different time intervals (0, 240 and 1440 minutes) at 5°C. Oviducts were collected from 9 adult female turkeys immediately after defeat. Samples were diluted and divided into four groups A (100% oviducts extract), B (50%), C (25%) and K (diluted only with saline solution) as a control. The measurements were carried using CASA - computer-assisted semen analysis. Similar values of progressive motility were found at time 0 minutes (values between 30.72% – K and 35.81% – B). After 240 minutes of culture considerable differences between the “oviducts” groups and control group were observed. The values in the all experimental groups generally increased compared to the control group. Similar results were detected after 24 hours of cultivation, but all values were decreased against 240 minutes of cultivation. Our work indicates that oviducts secret has positive effect on spermatozoa motility and viability. In all concentrations and time periods in experimental groups higher values of progressive motility compared to the control are reported.
turkey, spermatozoa motility, oviduct secretion, CASA