Laura Gjyli, Ariola Bacu, Jerina Kolitari, Silvana Gjyli
Microbiology of Microbiology
After isolation of phytoplankton DNA in coastal waters of Durres Bay, Albania, quantification and analysis of quality were investigated with spectrophotometric analysis. Analysis of UV absorption by the nucleotides provides a simple and accurate estimation of the concentration of nucleic acids in a sample. This method is however limited by the quantity of DNA and the purity of the preparation. Also biotic environment factors as Chlorophyll a and abiotic environment factors as temperature, salinity, pH, dissolved oxygen, turbidity, nitrate, phosphate were investigated to assess DNA quantities in different environment conditions. The Chlorophyll a was studied also to access the level of trophy. The sample stations were: Golem Beach (GB), Channel of Plepa (ChP), Hekurudha Beach (HB), Ex-Fuel Quay in Marine Durres Harbour (EFQ), Water Channel of Durres City (WChDC) and Currila Beach (CB). Samples are taken in one meter depth from the water surface. Water samples were collected monthly from April to October 2011. The most abundant stations with phytoplankton DNA are Channel of Plepa and Water Channel of Durres City. This confirms that there are spills of fresh waters, sewage or agricultural water spills, often discharge in coastal waters. Referring Mutliple Regression Analysis and single regression analysis, the association between phytoplankton DNA and environment factors was strong (R2 = 0.75). Basing in single correlation and statistically significance (p-value ≤ 0.05), the enviroment factors that correlated to phytoplankton DNA were pH, salinity and phosphate; explaining thus the variation of total phytoplankton in Durres Bay coastal waters.
Phytoplankton DNA, Chlorophyll a, environment factors, trophy