APOPTIC ACTIVITY OF CHICKEN ANEMIA VIRUS VP3 GENE CLONED IN REPLICASE BASED EUKARYOTIC VECTOR

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December – January 2013/14, vol. 3, no. 3
pages: 240-242
Article type: Biotechnology of Biotechnology
Abstract: CAV is one of the smallest avian viruses; it is 23–25 nm in size, icosahedral in shape and non-enveloped, having a 2.3 kb, circular, single-stranded, negative sense DNA genome. The genome encodes three viral proteins VP1, VP2 and VP3, that are transcribed from a single major transcript of 2.0 kb. The apoptosis inducing potential of CAV-VP3 (apoptin) was studied in HeLa cell line. The study confirmed that VP3 induced apoptosis in HeLa cells, which was confirmed by demonstrating the characteristics changes of apoptosis which include nuclear condensation, DNA fragmentation by DNA laddering assay, plasma membrane alteration by annexin-V binding assay and Caspase 3. The objective of our work was to study the apoptic activity of apoptin in HeLa cell line.
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