PURIFICATION AND CHARACTERIZATION OF SOLVENT STABLE LIPASE FROM A SOLVENT TOLERANT STRAIN OF GEOBACILLUS STEAROTHERMOPHILUS PS 11

Back to full issue:
June – July, 2016, vol. 5, no. 6
pages: 602-605
Article type: Biotechnology of Biotechnology
DOI: 10.15414/jmbfs.2016.5.6.602-605
Abstract: An extracellular organic solvent stable lipase produced by solvent tolerant strain of Geobacillus stearothermophilus PS11 was purified and characterised. The overall purification was 8.04 fold with a yield of 22.6%. The molecular weight of purified lipase was approximately 27.5 kDa. The purified lipase activity was stable (745 EU/ml at 72h incubation) in presence of toluene, benzene, propanol, methanol etc. The enzyme activity was maximum (764 EU/ml) when assayed under optimum temperature and pH of 50⁰C and 10.0, respectively. The enzyme showed stability at a wide range of temperature from 10⁰C to 60⁰C. This solvent stable lipase can be a novel tool for biodiesel industry.
XMLs: | NLM DTD xml | Copernicus xml |
Full text pdf download link: Issue navigation: June – July, 2016, vol. 5, no. 6:
prev. article |p. 599-601| next article |p. 606-611|
Embed fulltext PDF: