IN VITRO PRODUCTION OF MICRORHIZOMES IN GINGER (ZINGIBER OFFICINALE ROSCO)

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October – November 2014, vol. 4, no. 2
pages: 142-148
Article type: Biotechnology of Biotechnology
DOI: 10.15414/jmbfs.2014.4.2.142-148
Abstract: The present investigation was carried out to highlight an effective protocol for in vitro production of ginger microrhizomes. Microrhizomes were induced at the base of the in vitro derived shoots upon transfer to MS medium containing various concentrations of (30, 60 and 90 g/L), BAP: 6-benzylaminopurine (3, 6 and 9 mg/L) and grown under varying photoperiodism in addition to the MS medium supplemented with 9 mg/L BAP and 60-90 g/L sucrose under 16-h photoperiod within 10 weeks of cultivation were the best conditions for microrhizomes induction. Ginger microrhizomes formation in vitro was found to be controlled by many factors, including the concentrations of BAP and sucrose as well as photoperiodism during culturing period.
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