The consortia of micro organisms obtained from contaminated food (Milkfed Verka Kheer) was effectively tested against antibacterial agents, i.e. Sodium benzoate, grinded grape fruit seed powder GSP, Lysozyme and EDTA by agar diffusion method. Bacterial inhibition by combinations using two levels from each of the three factors (EDTA, Sodium benzoate and Lysozyme) without AM films was evaluated using liquid incubation method. The levels of the agents were selected according to their permissibility standards. Statistical analysis of experimental data for their antimicrobial spectrum was carried out by multi regression analysis and framed poly-quadratic equation using coded factors and percentage contribution of antimicrobial agents was determined using Design-Expert software. Properties such as thickness, opacity, transparency, UV absorbance and efficient working pH of the film were also determined. The best result was observed with EDTA: Sodium benzoate: Lysozyme at 100 mM: 100 mM: 1000 IU respectively at pH 9, where maximum zone of inhibition was observed that is 21mm. No zone of inhibition was observed using GSP as antibacterial factor limiting its widely supported usage. The work screened was imperative in performing optimization studies for the combination treatments to incorporate in starch-glycerol based active packaging film.
Active Packaging, Antimicrobial Activity, Food Preservation, Response Surface Methodology