CHARACTERIZATION OF DIARRHEAGENIC ESCHERICHIA COLI ISOLATED FROM RAW BEEF, MUTTON, AND INTESTINES SOLD IN OUAGADOUGOU, BURKINA FASO

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April – May, 2016, vol. 5, no. 5
pages: 470-474
Article type: Microbiology of Microbiology
DOI: 10.15414/jmbfs.2016.5.5.470-474
Abstract: Diarrheagenic Escherichia coli are zoonotic bacteria commonly present in animal gut. The aim of our study was to determine the prevalence of diarrheagenic E. coli isolated from raw meats and intestines in open markets of Ouagadougou, Burkina Faso. A total of 450 samples were collected from beef, mutton, beef intestine and sheep intestine, in respective number 175, 175, 50 and 50. Diarrheagenic E. coli were isolated by using standard microbiological methods and then Multiplex Polymerase Chain Reaction was used for characterization. Among the pathotypes, enteropathogenic E. coli was identified by serotypage (slide agglutination). A 30% (135/450) were E. coli. 30% (40/135) of E. coli strains provided the virulence genes. 14% of Shiga toxin producing Escherichia coli, 13% of shiga toxin producing Escherichia coli-enterotoxinogenic Escherichia coli, 1% of enteroaggregative Escherichia coli, 2% of enteroinvasive Escherichia coli and 1% of enterotoxinogenic Escherichia coli. 41% (55/135) were enteropathogenic Escherichia coli belong to serotypes: (5%), O119 (3%), O127 (16%), O125 (9%), O126 (18%), O128 (5%), O114 (5%), O124 (5%), O142 (7%). This study show contamination of slaughter animal with diarrheagenic E. coli pathotypes in Burkina Faso. Precaution can take of mutation breeding level.
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