ETHANOL PRODUCTION AND SUGAR CONSUMPTION OF CO-CULTURE Saccharomyces cerevisiae FNCC 3012 WITH Candida tropicalis FNCC 3033 IN MEDIA CONTAINING INHIBITOR FERMENTATION

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October – November, 2017, vol. 7, no. 2
pages: 160-167
Article type: Microbiology of Microbiology
DOI: 10.15414/jmbfs.2017.7.2.160-167
Abstract: Inhibitor fermentation is one of the problems that arise in the ethanol production from lignocellulose waste. This work examined the ethanol yield and sugar consumption of mono and co-culture S. cerevisiae with C. tropicalis in media containing inhibitor fermentation. Furfural and phenol were used for inhibitor fermentation in basal medium with concentrations of 2.0 and 5.0 %, respectively. The basal medium contained 20 g.L-1 glucose, 20 g.L-1 D (+) xylose, 20 g.L-1 arabinose, 4 g.L-1 urea, 3 g.L-1 NaNO3, 3 g.L-1 NH4NO3, 1 g.L-1 KH3PO4 and 0.7 g.L-1 MgSO4·7H2O with pH adjusted to 5.5 with 1 mol.L-1 HCl. After furfural or phenol addition separately, and inoculation by mono and co-culture S. cerevisiae FNCC 3012 with C. tropicalis FNCC 3033, all media were incubated at 28–29 °C, 50% r.h. in the dark for 5 days in a rotary incubator at 60 rev/min. We found yeast colony count, sugar consumption, ethanol yields and efficiency of fermentation by co-culture S. cerevisiae with C. tropicalis higher than mono-culture S. cerevisiae or C.tropicalis in the fermentation media with or without inhibitors. This work indicated that co-culture S. cerevisiae with C. tropicalis were more tolerant to furfural and phenol. Ethanol yield 8.52%, 5.37% and 3.83% obtained from basal medium, basal medium plus 2.5 or 5.0 % furfural, respectively and efficiency of fermentation 27.00%, 17.00% and 12.20%. Ethanol yields 8.13%, 5.62% and 3.19% obtained from basal medium, basal medium plus 2.5 or 5.0 % phenol, respectvely and efficiency of fermentation 28.20%, 20.00% and 14.00%. Co-culture S. cerevisiae FNCC 3012 with C. tropicalis 3033 demonstrated potential as a fermentation process for ethanol production from lignocellulosic media content inhibitors. The use of this co-culture effectively utilizes hexose and pentose sugars in the substrate, increasing the yield and efficiency of fermentation for ethanol production.

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