STRAIN IMPROVEMENT THROUGH MUTAGENESIS AND OPTIMIZATION OF PROTEASE PRODUCTION BY ASPERGILLUS TERREUS CJS-127 USING JATROPHA SEED CAKE AS SUBSTRATE

Back to full issue:
October – November, 2017, vol. 7, no. 2
pages: 174-180
Article type: Microbiology of Microbiology
DOI: 10.15414/jmbfs.2017.7.2.174-180
Abstract: Jatropha curcas seedcake (JSC), a byproduct of the biodiesel industry was utilized for the production of protease. To enhance the protease production, the parent strain of Aspergillus terreus CJS-127 subjected to UV (15, 30, 60, 90, 120 and 150 min) and chemical mutagenesis by using ethyl methanesulphonate (EMS-125, 250 and 500 mg). Two mutant strains designated as UV-6 and EMS-5 selected for optimization of protease production using JSC medium by submerged fermentation. Under the optimized conditions of pH 7.0, temperature 30°C, incubation period 72 h, inoculum 105/ml spores, 150 rpm agitation, JSC at 2% level, the maximum protease production was 172.32 and 163.57 U/ml for EMS-5 and UV-6 mutant strains respectively. The addition of maltose (2%) was found to enhance the protease production in the mutant strains. The protease activity increased by 2.30 and 2.41 folds for UV 6 and EMS 5 mutant strains after optimization of cultural conditions.
XMLs: | NLM DTD xml | Copernicus xml |
Full text pdf download link: Issue navigation: October – November, 2017, vol. 7, no. 2:
prev. article |p. 168-173| next article |p. 181-185|
Embed fulltext PDF: