Cereals are main part of human nutrition and strategic resources. Thousands of barley varieties are used in food industry and especially for the production of malt in the brewing industry. In the present study, we were focused on utilization of SSR markers for differentiation and characterization of different barley genotypes. Barley collection was analyzed by 4 pure and 1 compound markers. Using STMS method we have revealed significant polymorphism. A total of 27 alleles were detected among 24 varieties with an average of 5.4 alleles per locus. Average values for index of diversity (DI), polymorphic information content (PIC) were 0.767, 0.756 respectively. The highest level of polymorphism was detected with SSR marker Bmag 0222 (7 alleles) which also revealed one heterozygous variety. Dendrogram was created by hierarchic cluster analysis using UPGMA algorithm on the basis of detected alleles. Analyzed genotypes were divided into three clusters so it can be used to study genetic relations among collection of analyzed barley varieties.
Barley, Genetic diversity, Simple sequence repeat (SSR), DNA marker