OPTIMIZATION OF CULTURE CONDITIONS FOR PRODUCTION OF BACERIAL CHITINASE ISOLATED FROM MARINE CRUSTACEAN SHELLS

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February – March 2014, vol. 3, no. 4
pages: 319-321
Article type: Microbiology of Microbiology
Abstract: Six bacterial isolates identified from marine crustacean shell wastes’ were studied under different growth parameters to optimize the production of chitinase. The enzyme activity was determined with different growth media and optimized under different conditions of pH, Temperature and substrate concentration. The enzyme activity was found to be maximum in nutrient broth with 0.3% colloidal chitin. The optimum pH and temperature were found to vary between the isolates. Under optimized condition, the chitinase activity was doubled than in the normal condition.
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