Ivan Malík, Rodney Govender, Eva Sedlárová, Jozef Csöllei, Josef Jampílek, Aidan Coffey, Jim O´Mahony
Microbiology of Microbiology
In a continual research of ortho-/meta-/para-alkoxyphenylcarbamic acid esters as antimycobacterially active compounds, the purpose of current paper was to in vitro screen the potency of the molecules containing incorporated substituted N-phenylpiperazin-1-yl moiety against attenuated M. tuberculosis H37Ra strain which was grown in Middlebrook broth, supplemented with Oleic-Albumin-Dextrose- Catalase supplement and mycobactin J (2 µg/mL) as well. The susceptibility of concerned mycobacterial strain was investigated in a 96-well plate format, the plates were incubated at 37°C for 7 days. According to minimum inhibitory concentrations, as the lowest concentrations which prevented a visual colour change, it was observed that positional ortho-/meta-/para-alkoxy side chain isomerism as well as high lipophilicity did not decisively influenced the effectiveness of inspected compounds. Moreover, qualitatively different substitution within basic compartment in terms of electronic properties and possible hydrophobic interactions did not lead to the improvement in the activity.