Matej Brestenský, Soňa Nitrayová, Peter Patráš, Jaroslav Heger
Biotechnology of Biotechnology
The six cannulated gilts (initial body weight 35.8 ± 0.5 kg) fitted with a T-cannula in terminal ileum, were used to determine the apparent (AID) and standardized (SID) ileal digestibility of nitrogen (N) and amino acids (AA) in broken rice. Animals were fed twice daily in a two equal doses at a daily rate of 80 g.kg - 0.75. Water was offered ad libitum. The tested feed was the sole source of protein in the diet. The N-free diet was used to determine the ileal endogenous flow of AA and N. Chromium oxide (Cr2O3) was added to the diets as an indigestible marker in an amount of 0.3 % per kg of diet. After a 14 d postoperative period a 6 d adaptation period followed during which the animals were fed with an experimental diet. On d 7 ileal digesta was collected continuously for 24 h. The AID and SID of AA and N were calculated using analytically determined values of N, Cr2O3 and AA. The SID of AA was in a range from 81.6 % (tyrosine) to 112.6 % (proline) (P < 0.05). Ileal digestibility for lysine, alanine and leucine were 94.1, 94.3 and 94.7 % (P > 0.05), respectively. There were no differences between standardized ileal digestibility of essential amino acids (94.3 %) and nonessential amino acids (95.3 %). Regarding the ileal digestibility of AA, broken rice, a by-product from the food industry, is an appropriate source of digestible AA for growing pigs.