Antifungal activity of the Bioxil containing hydrogen peroxide as an active agent has been studied. The mentioned species act as indicators of microbiological quality of fruit juices and hygienic condition of juice processing industry. Inhibition activity of the mentioned disinfection agent has been studied in vitro against different yeast strains: Rhodotorula rubra J-120 and Saccharomyces cerevisiae J-200 and their mix culture. For the evaluation of antifungal efficacy of the Bioxil suspension and surface tests were used. Relation between antifungal activity of Bioxil, its concentrations, yeast species and contact times was established. Bioxil containing 1% and 3% of H2O2 has shown unequal inhibition activity against different yeast strains. Influence of washing and disinfection steps on the level of contamination of conveyor and working surfaces by yeasts and has been investigated. In the processing conditions, after treatment of conveyors and working surfaces, without preliminary washing, lower inhibition activity of the Bioxil has been occurred.