Abstract: This is the first report about the occurrence of microscopic filamentous fungi in plant pollen. In our study was analyzed plant pollen of dogwood common (Cornus mas), ray mountain (Secale strictum spp. strictum), pumpkin rape (Cucurbita pepo var. styriaca) and vines (Vitis vinifera). Thirty two samples of plant collected pollen were gathered from trees and plants. The samples were examined for the concentration and identification of microscopic fungi able to grow on Malt and Czapek-Dox agar. The microscopic fungi isolated from dogwood common pollen ranged from 3.12 to 4.93 log CFU.g-1. The microscopic fungi isolated from ray mountain pollen ranged from 0.00 to 3.51 log CFU.g-1. The number of microscopic fungi from pumpkin rape pollen ranged from 0.00 to 3.00 log CFU.g-1 and in vines pollen ranged from 2,52 to 4.34 log CFU.g-1. In plant pollen samples we found the representation of 7 genera and 19 species of microscopic fungi. From plant pollen samples, 138 isolates were recovered. The most frequently species in the plant pollen samples was Alternaria alternata (19 isolates), Mucor racemosus (11 isolates), Mucor mucedo (33 isolates) and Cladosporium cladosporoides (10 isolates).