Abstracts Special issue on Animal Physiology 2013, vol. 2, Abstracts special issue
Alexander V. Makarevich, Eliška Špaleková, Luděk Stádník, Elena Kubovičová, František Louda
Biotechnology of Biotechnology
A number of factors influencing bull fertility are identified including scrotal circumference, sperm morphology, motility, mating ability, age, body condition and the breed of the bull. This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. Samples of ejaculate were obtained from each bull using an artificial vagina. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement ≥70% were diluted in AndroMed® extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-1 in combination with propidium iodide (PI), using specific wave-length filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0.05) in CF (2.84±0.17) than in H (2.19±0.13) bulls. Total sperm motility and progressive movement measured by CASA immediately after thawing (38.6±1.8 and 34.19±1.91, resp) and following 0.5 h (46.44±1.79 and 44.14±1.8) were higher in Holstein bulls compared to CF bulls (34.15±2 and 30.27±2,13; 39.17 ±2 and 36.12±2.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41±1.81%) compared to H bulls (26.33±1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12±1.71%) compared to H bulls (12.58±1.51%). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. These results should be taken into account at preparation of insemination doses. This study was supported from the grant agencies of Slovak Republic (APVV-0137-10) and Czech Republic (NAZV QI91A061).