THE EFFECT OF HT-2 TOXIN WITH YUCCA (YUCCA SHIDIGERA) ON SECRETION OF STEROID HORMONES BY PORCINE OVARIAN GRANULOSA CELLS

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Abstracts Special issue on Animal Physiology 2013, vol. 2, Abstracts special issue
pages: 16-17
Article type: Biotechnology of Biotechnology
Abstract: The aim of this in vitro study was to examine the secretion of progesterone, testosterone and estradiol by ovarian granulosa cells after addition of HT-2 toxin in combination with yucca. Ovarian granulosa cells were incubated withouth (control) or with treatments at the doses 1, 5 and 50 ng.ml-1 of HT-2 toxin plus yucca 50 µg.ml-1 for 48h. Progesterone, testosterone and estradiol were determined by RIA. After addition of 5, 50 ng.ml-1 (but not at 1 ng.ml-1) of HT-2 toxin with yucca 50 µg.ml-1 the release of progesterone was significantly (P < 0.05) inhibited. The release of testosterone was significantly (P < 0.05) inhibited by 1 ng.ml-1 (but not at 5 and 50 ng.ml-1) of HT-2 toxin with 50 µg.ml-1 of yucca. We observed significant (P < 0.05) stimulation in estradiol release in all experimental groups. There is a little information about the effect of A-trichothecenes on secretion activity of animal ovarian granulosa cells. The results of our in vitro experiments indicate the possible dose-dependent effect of HT-2 toxin with yucca on secretion activity to release steroid hormones.
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