The main objective of the current research work was to investigate the physicochemical properties of ice samples collected from the snout of three important glaciers located in the Garhwal Himalaya along with the psychrophilic microbial diversity. This study was carried out for two years from 2015 to 2016 with two sampling attempts each year. Ice samples were collected from all the pre-identified sampling sites at a depth of 5cm to observe and record the characteristics and to isolate and identify the psychrophilic diversity of microorganisms. Overall fourteen physical and chemical properties of ice samples were recorded. The psychrophilic microbial diversity was identified through the morphological, biochemical and MALDI-TOF-MS techniques. The α–diversity of microbes in the ice of Satopanth glacier was 17 with 08 bacterial species, 04 actinomycetes species and 05 fungal species. However, the α–diversity of microbes in the ice of Bhagirathi-Kharak glacier was also 17 with 06 bacterial species, 05 actinomycetes species and 06 fungal species. The α–diversity of microbes in the ice of Gangotri glacier was 19 with 09 bacterial species, 05 actinomycetes species and 05 fungal species. The present research work can be a decent reference for additional investigations in a similar discipline. The available data will also help the researchers to study the psychrophiles, their existence in glaciers and their importance to society.