RESPONSE OF CADAVERINE ON THE PROTEIN PROFILING OF CULTURED TISSUES OF BRASSICA JUNCEA (RH-30) UNDER MULTIPLE STRESS
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June – July, 2021, vol. 10, no. 6
Pushpa C. Tomar, Komal Arora
Biotechnology of Biotechnology
A lysine catabolite named cadaverine, a polyamine is known as plant growth regulating compound. Its precise role as growth regulators and/or inducer of nitrogen assimilation positively affects plant growth and productivity under different stress condition. Cad strongly maintains the stressed plant growth through maintaining the metabolites and ionic homeostasis. This entry summarizes not only cadaverine effect on B. juncea callus in terms of the growth parameters but also on protein content and profiling of these mustard calli. The basal medium used for tissue culture was MS media augmented with different stress conditions and treatments (100mM NaCl, 1mM Cd or Pb, 1mM cadaverine or 5mM NH4NO3). Better growth was observed in callus grown in media supplemented with cad as compared to control and stressed one. Also, callus sub cultured in cad media maintained desired growth of callus over control and even with NH4NO3 supplementation. The application of Cad suppressed the accumulation of 66 kDa peptide under salt stress whereas the same peptide’s expression remained unaffected by NH4NO3 supplementation. NH4NO3 induced the expression of 54 and 44 kDa peptides under NaCl and Pb stress. Sub- cultured callus upon Cad supplementation significantly expressed peptides of 76, 72, 45, 42, 40, 32, 30, 24, 22 and 21 kDa, where expression of 66 and 34 kDa peptides were prominent. Cad boosted the callus formation and growth when grown in stressed conditions and these stress induced proteins in the presence of Cad suggesting their role in the growth of plant under various stress conditions.
Brassica juncea; Cadaverine; Callus; Polyamines; Protein; Stress
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