Róbert Toman, Mária Adamkovičová, Peter Massányi, Michal Cabaj, Norbert Lukáč, Monika Martiniaková, Radoslav Omelka
Biotechnology of Biotechnology
The present study was carried out to elucidate the effects of heavy metal cadmium (Cd) and a nonselective organophosphorus insecticide diazinon (DZN) administration on the testis structures in adult male rats in subchronic toxicity experiments. A total of 40 Wistar 4 weeks old rats were randomized into 4 groups of 10 animals each and dosed with cadmium (Cd) 30 mg/l (group A), diazinon (DZN) 40 mg/l (group B), and in combination of Cd and DZN (30 and 40 mg/l, respectively, group C) per os in drinking water for 90 days. Testicular histology using a light microscopy and morphometry using PC morphometric software M.I.S. Quick Photo were evaluated. The morphometric data supported histological observations at tubular and interstitial level. Reduced seminiferous epithelium (P<0.001) in Cd group showed desquamation of germ cells, cellular degeneration and necrosis. Increase in epithelial vacuoles and dilated blood vessels relative volume (P<0.001) were observed in all experimental groups. Cd and DZN and their combination exerted changes in the reproductive parameters which could be subsequently negatively related to male fertility. These data provide a novel insight into the reproductive toxicology of Cd-DZN in male rats. However, results did not indicate synergistic or additional effect of simultaneous administration of both toxicants.
testis, histology, morphometry, rat, cadmium, diazinon, fertility